Hi

I am new to scRNA seq and am interested to know how to find the threshold for number of genes that i should expect in single cells. I am getting wide range from ~1600 per cell to around 4000 per cells. I am already considering cells with more than 2500 genes to be doublets. Most of my cells have less than 2000 genes. IS there any way how i can decide the threshold and to exclude the cells with more genes:

Methods: Smart-seq2

I decided to call gene present or absent if TPM>1.

Any help in this regard would be deeply appreciated.

Regards