DNA Integrity in Whole Genome Sequencing
How important DNA integrity in the whole genome resequencing? If the early step of the library construction is DNA fragmentation (sonication), why NGS companies reject the sample that has degradation, or fragment size lower than 20kb?
• 1,706 views
•
link
written
by
rizky_dsatrio •
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
Whole genome resequencing sample size for population genetics?
written by Dylan •
-
For people doing high molecular weight DNA sequencing library prep, do you see a steep drop off in …
written by Mark •
-
I have a question in Size-selection(SPRI) in ChIP-seq library preparation
written by oryzae51 •
-
Illumina technology output, 16S amplicon
written by Jelo •
-
Tool: ctDNAtools: an R package to work with ctDNA sequencing data
written by amjad
-
Library Prep for NGS
written by Sujata •
-
MACS for chip-seq: difference between sonication size(bandwidth) and fragment size?
written by salamandra
-
Is the consistency of the NGS library size important?
written by mrs.hope •
-
Discrepancy between agarose gel and bioanalyzer after sonication for ChIP
written by augelloma •
-
Forum: Quantifying ChIP-seq data: a spiking method providing an internal reference for sample-to-sa…
written by Sukhi Singh
I believe companies do not "reject", they probably warn the client the quality of sequencing and downstream analyses may be negatively affected. If the client then chooses to proceed, companies will proceed.
Dear h.mon
Thank you for your comment. How do you think about the downstream analysis that may be affected by low integrity DNA? If sonication step produces fragmented (small-size) DNA, why low integrity DNA can affect the sequencing quality or downstream analysis?
Regards