Hi, community,
I have done my amplicon 16S illumina miseq sequencing. My samples were from wasterwater. However, the raw reads were only around 2000~10000. Is that right?
May I know is there anything wrong with my data?
Thanks in advance.
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luyang1005 •
That's strange!
How do you calculate that "2000~10000" ?
Hi, 2000~10000 means the raw reads count for each fastq file. But my reads phred score are all above 30. May I know is this right? Thanks.
I know thats the number of reads. What I wanted to know is how do you count the number? I want to make sure that there is no mistake in counting; although this is a trivial task.
I use the fastqc and do the fastqc, it reported to me the result reads number
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