Reversal of cross-linking cause DNA loss in ChIP-Seq
Hello Biostars!
This is more like 'ChIP-Seq protocol' question.
Summarized protocol for ChIP is here: https://en.wikipedia.org/wiki/Chromatin_immunoprecipitation
It has been mentioned after immunoprecipitation of the protein-DNA complex , protein–DNA cross-link is reversed and proteins are removed by digestion with proteinase K.
Can anyone tell me if during reversal of protein-DNA complex the DNA at site of cross-linking is included in further sequencing step or is washed away with the protein itself?
Please help. Basics of ChIP-Seq links will also work. Thank you :)
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Hi Genomax. I thought if the question is related to NGS, it is appropriate to ask people here.