This is a test version of Biostars. For the public version, visit https://www.biostars.org.
What is the exact formula for the calculation of Fold Change (FC), p-val and q-val in Ballgown stattest?

I am looking for the exact formula for the calculation of Fold Change (FC), p-val and q-val in Ballgown stattest?

By using gexpr I got the gene expression values for the list of genes. For better understanding I tried to calculate the FC for a few genes manually but the values I obtained differed from the values given by stattest. Can you please help regarding this?

rna-seq ngs foldchange

Gene exprs value which you got is in logged scale or unlogged scale. If it is logged scale then you need to check manually using formula Test-Control because log((Test)/(control)) will be log(Test)-log(control). If it is unlogged scale the Test /Control is used to calculate FC.

0 answers

No answers yet.

Log in to answer this question.