I want to combine three microarray datasets (E-TABM-158, GSE2034, and GSE3494) all of them from the same platform(HG-U133A). However, the raw data were normalized by RMA, MAS5, and global mean method. I want to find Fold change value for the combination set. Is it enough to use z-score before combine them and then calculate the FC value by this formula:
if((group2_mean(k)/group1_mean(k))>=1)
fc=(group2_mean(k)/group1_mean(k));
else
fc=-(group1_mean(k)/group2_mean(k));
end
Thank you in advance
•
written
by
lur_murad •
Yes, I have Series Matrix File as text file which contain the intensity value for each prop. Do you mean I have to download the cell files and normalize them in the same normalization method? excuse my question I am new in this area
Yes, I would recommend downloading the cel files, loading these files in R, then using the R program LIMMA to normalize each dataset using the same normalization method and then perform your analysis. There may be better programs than LIMMA but if I recall correctly you should be able to do the data normalization and analysis with this bioconductor package.
Log in to answer this question.
