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Refseq human reference transcriptome link for RNA-seq differential expression analysis

Hi there, I'm trying to find the right Refseq reference transcriptome link to use as an index for kallisto. I've been looking on the NCBI FTP site but not sure where to go from ftp://ftp.ncbi.nlm.nih.gov/refseq/H_sapiens/. Can anyone help?

rna-seq alignment
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written by Abiterkuile • Abiterkuile

GENCODE releases are the official location of human genome data. If you only need the transcripts then look for that fasta file.

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written by GenoMax GenoMax

Unless you have a particular reason for using RefSeq I would not recommend using it with Kallisto since Kallisto obtain it's accuracy from the transcript quantification - and Refseq have very limited transcripts. Use Gencode as suggesed by Genomax. Else you can find RefSeq via the table browser

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written by Kristoffer Vitting-Seerup Kristoffer Vitting-Seerup

If you must have RefSeq versions then take a look at the last column of this file. It contains the accession numbers of the genes you want. You could then use NCBI eutils to get the fasta sequence.

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written by GenoMax GenoMax

To get the sequence of RefSeqGenes as fasta using NCBI eUtils.

awk -F '\t' '{print $4}' gene_RefSeqGene | sed -e '1d' - > genes
for i in `cat ./genes`; do efetch -db nuccore -id $i -format fasta; done
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written by GenoMax GenoMax

If you have no particular reason to go for RefSeq, I would suggest you the same thing that was suggested to me by a Kallisto author in june 2017 :

Anyway, our links would simply point you to the ensembl transcriptome when it exists. We typically use this as it is fairly complete (by comparison with others) and the transcript to gene name mapping is pretty nice (also by comparison with others).

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written by erwan.scaon erwan.scaon

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