demultiplexing with same indices across different lanes
When demultiplexing, is it a problem if same indices are used across different lanes?
For example:
lane 1 has 4 samples with indices A1, A2, A3 and A4
lane 2 has 4 samples with indices A1, A2, A3 and A4
As far as I know, demultiplexing is done per lane? so there should be no problem in this?
1 answer
That is correct. There should be no problem. I assume you are referring to the same indexes being used for different samples.
Note: This will not work with NovaSeq (without XP add-on) or NextSeq series of sequencers. Even though there are optically separate lanes on FC for these sequencers they are fluidically fed by the same sample pool across the entire FC.
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Hello lait!
We believe that this post does not fit the main topic of this site.
Not really bioinformatics
For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.
If you disagree please tell us why in a reply below, we'll be happy to talk about it.
Cheers!
why this is 'not really bioinformatics' ? demultiplexing is done by running a script. so there is some bioinformatics involved. bcl2fastq is the name of the demultiplexing script, and there have been many questions about this topic.
Alright, I'll reopen it now. Apologies!