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tools for polyploid physical phasing

I have >100x HiSeq reads for an organism, and I want to determine sequences of each copy of a tandem duplicated gene cluster.

I have done it as follows:

  • mapping reads to reference gene (BWA)
  • estimating the copy number based on sequence depth (samtools)
  • calling variants with GATK HaplotypeCaller -ploidy n
  • physical (read-based) phasing by my hands like solving SUDOKU or logic puzzles

Yes, I did it. But I wonder there must be sophisticated tools for the last step.

Any suggestions?

physical phasing polyploid

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