estimate error profile from aligned Illumina reads
Hi folks, Can anyone recommend a tool that can loop through a BAM file and give me information about which base substitutions are present at each position in the reads?
Ideally, it would report a table that would give the estimate error rate at each read position.
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Hi Richard,
RSeQC's mismatch-profile can do this. The result table is stored in a tab-separated file which has the misleading extension xls.
Cheers
Michael
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