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Gene expression Box Plot

Hi,

I have the below box plot for gene expression, please could you help me with meaning of it

https://ibb.co/meKS76

Thank you

gene

@Kevin, Thank you, it is raw data no normalization. when I normalized it looks like:![enter image description here][1]

https://ibb.co/cu4AS6

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1 answer

It says the following:

  • The vast majority of your data points in each sample have values close to zero, as indicated by the fact that both the median bars and upper/lower quartile perimeters are scrunched up at the zero mark
  • There are many outliers in your samples, as indicated by the points outside the upper-quartile perimeter.

It does not look like you are plotting logged values. This looks like FPKM-normalised counts, or normalised counts on the negative binomial distribution.

Not that you can re-generate this without the outliers by specifying outline=FALSE to the boxplot function.

Kevin

@Kevin, Thank you, it is raw data no normalization. when I normalized it looks like:![enter image description here][1]

https://ibb.co/cu4AS6

The conclusion:

Normalisation successful ! :)

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