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GATK HaplotypeCaller error ?

Hi, I tried to generated a .VCF file using GATK HaplotypeCaller. I provided all the input requirements hg38.fa, hg38.dict, hg38.fa.fi and Bam file. Java 1.8 and picard

I'm getting this error, kindly reply.

Command : java -jar GenomeAnalysisTK.jar HaplotypeCaller -R /home/Downloads/GenomeAnalysisTK-3.8-0-ge9d806836/hg38.fa -I /media/CANCERGENOMICS/NA12878.bam -o /home/Downloads/GenomeAnalysisTK-3.8-0-ge9d806836/NA12878GATK.vcf

INFO 12:15:43,860 HelpFormatter - ---------------------------------------------------------------------------------- INFO 12:15:43,862 HelpFormatter - The Genome Analysis Toolkit (GATK) v3.8-0-ge9d806836, Compiled 2017/07/28 21:26:50

alignment genome

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PS: duplicate

1 answer

The chromosomes in the bam and the chromosomes in the reference genome don't match. Did you do the alignment or did you download the data from somewhere?

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