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microarray data analysis

hello, i am working on microarray data set and trying to find out differentially expressed genes. i have normalize microarray data using rma algorithm. i want to calculate fold change for selection of differentially expressed genes. rma algorithm give log2 transformed value, i am not confirm. then how i can calculate fold change?

microaaray
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updated by Jean-Karim Heriche Jean-Karim Heriche • written by au.rinki.bio • au.rinki.bio

FYI, Intensity values are log transformed in last step of RMA i.e summarization.

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written by cpad0112 cpad0112

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Most normalization algorithms (all ?) give log2 expression value 0-16. You can use these values to find differential genes implementing methods like limma. You might be interested to check following links

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written by venu venu

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