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Insert size is so small, good or bad?

If you have the average insert size is ~158 estimated using BBMap . While the read length from the fastq file is 150. so we paired reads length (2*150)

This means the paired end reads overlap by too much. Is this good or bad?

insert-size rna-seq
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updated by Ram Ram • written by Tania Tania

Bad, the insert size of my some bam files is one or zero. Then, I use the manta to call SV, it failed.

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written by Jingjingzhang • Jingjingzhang

1 answer

This is a related good read https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3906532/

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written by Sharon Sharon

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