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Best way to align RNA unaligned .ubam files from Ion Torrent

Hello,

Could someone please suggest a good way to align an unaligned Ion Torrent .ubam files containing RNA reads, so we could identify the breakpoints in IGV?

Thanks

next-gen

Use either samtools fastq, bedtools bamtofastq or picard samtofastq to get the reads in fastq format. From there on standard alignment procedures apply.

1 answer

Could you please suggest a pipeline for aligning RNA fastQ files, bam being the final output?

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