Best way to align RNA unaligned .ubam files from Ion Torrent
Hello,
Could someone please suggest a good way to align an unaligned Ion Torrent .ubam files containing RNA reads, so we could identify the breakpoints in IGV?
Thanks
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Could you please suggest a pipeline for aligning RNA fastQ files, bam being the final output?
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Use either samtools fastq, bedtools bamtofastq or picard samtofastq to get the reads in fastq format. From there on standard alignment procedures apply.