More posts like this
-
Question regarding MACS2 approach of peak calling
written by rkc5 •I know the way MACS2 uses Poisson distribution to find the p value for the enrichment of a signal peak(observed) as compared to the local …
-
How to Compare Our Control VS. Sample Peaks from CHiP Seq
written by screadore •If anyone could help me figure out how to compare the control vs sample peaks for CHiP Seq. I used Illumina's BaseSpace CHiP Seq app …
-
Identifying differential histone mark (H3k27me3 and H3k4me3) in treated and control sample
written by munaj86 •Hi,, I'm analysing chip seq data for histone mark h3k27me3 and h3k4me3. for each condition, I have two samples: treated sample and control sample. I …
-
Algorithmic Difference between MACS and MACS2
written by taraeicher •I think I have a pretty good understanding of how the MACS algorithm works. In a nutshell: 1. Remove duplicate reads. 3. Model a Poisson …
-
How is FDR calculated in MACS?
written by AlexAbdulkaderKheirallah<p>Hello All,</p> <p>My question concerns the calculation of empirical FDR by MACS peak caller. According to the authors "For a particular P value threshold, the …
-
Chips-Seq Replicates And Motif Discovery: What Is The Most Sound Way To Deal With The Merged Peaks …
written by Nick<p>I have chip-seq triplicate (3 treatment, 3 controls, each of the 6 with their input control). I have identified the peaks using macs14 for each …
-
Peak Finding Of Chip-Seq (Or Clip-Seq) And Poisson Distribution, Am I Right?
written by Puriney<p>Hi, Check my understanding towards how Poisson distribution is employed when finding peaks of CHIP-seq and CLIP-seq. It is well known that the number of …
-
Tweaking Findpeaks Output
written by darcangelo.elisa •<p>Hey everyone, </p> <p>I am analysing Chip-seq data with MACS and FindPeaksb (I am dealing with a transcription factor). Being new to the world of …
-
Standard Fdr Threshold In Chip-Seq & How To Co-Relate It To Q-Value [Macs14]
written by Sukhi Singh<p>Hi,</p> <p>My first question is, what is the standard measure of FDR (if any) while calling peaks in ChIP-Seq. </p> <p>And, how to co-relate the …
-
One Sided Peak Analysis
written by Bri •<p>Please can you suggest me of any tools available for one sided Chip Seq data analysis. I have a raw Chip-seq data but not a …
Hello Ben!
Questions similar to yours can already be found at:
We have closed your question to allow us to keep similar content in the same thread.
If you disagree with this please tell us why in a reply below. We'll be happy to talk about it.
Cheers!
I need to calculate the difference of two ChIP-seq, not one ChIP-seq and input
Please explain your experimental setting and data better in your question by editing it. What is the difference between doing case-control and case-background analysis?
Maybe, it is DiffBind what you are looking for