running DESEQ2 results in GSEA
I am trying to run my preranked rnk file (generated using the DESEQ results). I followed the instructions given in http://genomespot.blogspot.com/2014/09/data-analysis-step-8-pathway-analysis.html to generate it.
When I run the rnk file GSEA, I get the following error:
"After pruning, none of the genes passed size thresholds."
How do I troubleshoot this?
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Biostar
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lavanya.kannan •
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lavanya.kannan •
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It will be easy if you can provide the parameters used for the analysis.