Gallus gallus expressed proteins in the range 170 kDa to 10 kDa. Here are their molecular weights, please guide me how can i find their names by easy and fast way?
MW
(d)
92623
69470.6
67705.9
59411.8
38655.9
102903.2
94000
79230.8
71384.6
69464.3
64285.7
24942.5
17643.7
16609.2
14326.9
12596.2
•
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arjahejo •
search ncbi/protein using entrez field:
e.g. 92623 https://www.ncbi.nlm.nih.gov/protein?term=92623%5BMolecular%20Weight%5D
Short answer: You can't without additional information.
In a typical gel, a band is a mixture of proteins. Also the size resolution of a gel is not enough to unambiguously ID proteins. To complicate things, post-translational modifications also affect how a protein moves in a gel. One way of identifying what is in a band is to cut it out of the gel and analyze it's content by mass-spectrometry. Another way is to test candidates by Western blotting.
I have peptide sequences, now how can i find the protein molecular weight? Sepcie gallus gallus. Please reply i am very much desturb.
See my answer to your question here.
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You cant.
Run a mass spectrometry experiment with digests from your gel for peptide sequencing.
Also, not a bioinformatics question.
I run that but i have list of 90+ expressed proteins, i don't know which proteins have what molecular weight. I got that data from SDS-PAGE.
Molecular weight is unlikely to unambiguously identify proteins. Don't you have the peptide sequences?
No i don't. I have only SDS-PAGE results. Someone told me that we can find it with the help of String or Skyline.
So, you haven't run a proteomics experiment then? If you did an in-gel digest, you could get the protein sequences and work out what they are.
Someone may know something I don't but it seems very unlikely you can identify a protein on molecular weight alone. I certainly wouldn't trust that data if someone showed it to me.
Sorry i forget about it, i have peptide sequences, now how can i find the protein molecular weight? Sepcie gallus gallus. Please reply i am very much desturb.