Infer RNA-Seq library preparation from BAM
Dear all,
Do you know a tool, which gives information, if a RNA-Seq library is poly-A or ribosomal depleted? I received a lot of RNA-Data, which has been sequenced in different labs with different libraries. I could check the RNA-SeQC output for the coverage at the 3' and 5' ends, but this will take forever. And yes, I already asked Google...
Thanks guys!
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I will assume your data is human RNAseq: find some non-poly-A lncRNAs with reasonable expression, them use those to infer library prep.
Or ask the person who organised all this data if he have this information as well.