Mothur_already merged miseq_sequences_how can I use make.contigs?
Hi! Ìm using mothur when I have to qualify the paired end miseq raw sequences
Usually I made stability files and undergo make.contigs(files=stability.files) but
Recently I got data that already merged sequences (miseq paired end)
As you already know, input for a make contigs command, should be a stability.files that made of forward and reverse fastq
What should I do ??? with already merged fastq files ???
If there is no solution,,, I have to request raw data before merge but It should be a final way...
Somebody help..
• 1,489 views
•
link
updated
by
gb
•
written
by
yusookzzz •
•
written
by
yusookzzz •
1 answer
oh and I already use fastq.info() command
but it is not for a miseq paired end uses ...
• 0 views
•
link
written
by
yusookzzz •
Log in to answer this question.
More posts like this
-
Trimming pair end fastq files merged not interleaved
written by ricfoz •
-
over represented adapter in reverse but not in forward reads?
written by demoraesdiogo2017 •
-
removing seqs from paired end read amplicon dataset with mothur
written by lotta.purkamo •
-
How to split fastq cointaing shared forward barcodes but different reverse ones?
written by oscar.nvergara •
-
mothur - stability.files does not contain sample group names
written by pjdavis •
-
Mothur dist.seqs output
written by pkozyrev •
-
How do i should proceed with 16S rRNA amplicon sequencing data from Illumina MiSeq using QIIME pipe…
written by antonioggsousa
-
running Tophat on merged reads
written by rob.costa1234
-
16s amplicon metagenomics - no mapping file?
written by manekineko
-
How to deal with demultiplexed Miseq pair-end (2*250bp) 16S data using QIIME?
written by nkuyfq
