Is it possible to manipulate Chromatogram ?

Is there a way to manipulate Chromatograms peaks and sequences? So that we can see better heterozygosity and better genotypes

chromatogram sanger abi fastq

• 1,901 views • link

updated 3.4 years ago by Ram

• written 8.9 years ago by Clark_BioMorgan •

Most .ab1 file viewers should allow you to do that. You are only referring to manipulating the display of the chromatograms, correct? Give NovoSNP a try.

• 0 views • link

written 8.9 years ago by GenoMax

0 answers

No answers yet.

More posts like this

filtering heterozygous genotypes
written 1.5 years ago by Bushra Bibi

I have merged vcf file. I need to keep heterozygous genotypes with heterozygosity < 0.2. How can I do through bcftools? I found this command …
R package to visualize multiple aligned chromatograms
written 2.0 years ago by DaniCee •

I have 5 ab1 files, and I know how to create a chromatogram in R for each one of them individually, using `sangerseqR`. seq_obj <- …
GWAS and Hetrozygosity
written 4.5 years ago by Star:) •

Hi everyone, I have a question. Is there a relationship between heterozygosity and GWAS manhattan plot? For example, we have two populations that have low …
how to filter out all the homozygous genotypes in a multi sample VCF file?
written 5.1 years ago by Nahid Bandi K •

Hi all, is there way to filter out all the homozygous genotypes in a multi sample VCF file? in my vcf file I have unknown …
comparing distinct peaks from riboseq profiling results
written 5.3 years ago by Assa Yeroslaviz

Is there a way to compare peaks from two different conditions of a ribosomal profiling experiment similar to a ChiP-Seq analysis? Can one use for …
Sanger sequencing chromatogram aligned to reference gene
written 5.6 years ago by vujex •

I did Sanger Sequencing on 10 samples targeting one specific gene to see whether we detect a particular SNP. We did forward, reversed and tested …
Annovar annotation with refGene Database How to Dismiss Exon number and Gene Symbol from AAchange C…
written 8.5 years ago by Clark_BioMorgan •

Dear all, I use annovar annotation with refGene database as gene annotation. In the output column AAchange the format is like this; GeneSymbol:Transcript:ExonNo:baseChange:AAchange But I …
calculate Per variant Heterozygosity from VCF file
written 8.6 years ago by SOHAIL

Hi everybody, Is there any way to calculate the per variant heterozygosity (i.e. number of 1/0 or 0/1 genotypes observed at given variant site for …
calculate Per variant Heterozygosity from VCF file
written 8.6 years ago by SOHAIL

Hi everybody, Is there any way to calculate the per variant heterozygosity (i.e. number of 1/0 or 0/1 genotypes observed at given variant site for …
Suggestion for de novo transcriptome assembly of a plant with high heterozygosity
written 10.9 years ago by seta

<p>Hi all,</p> <p>I've got Illumina sequencing reads (100 bp, PE) from a non-model dioecious plant (two genotypes, one is sensitive to treatment and another is …