Normalizing Human Samples in .bam format
Hello All,
On going through some R packages like DeSeq2 for normalizing data I couldn’t get a normalized output. I would like to know if there is any other alternate way to normalize these human samples which are from Illumnia sequencing platform, paired-end data and in .bam format.
Thank you
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Devon Ryan
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mittu1602
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mittu1602
1 answer
If "normalized output" is meant to mean "normalized counts", then in DESeq2 it's counts(dds, normalized=TRUE). If you're looking for something else then you need to clarify exactly what you want.
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Devon Ryan
Average the normalized counts then (presuming whatever you're doing accepts that).
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