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ERROR: File supplied on command line; use -s, -pacbio-raw, -pacbio-corrected, -nanopore-raw, or -nanopore-corrected

Hi everyone, I have tried to run the tutorial on ecoli supplied on the CANU readthedocs http://canu.readthedocs.io/en/latest/quick-start.html. I downloaded the supplied pacbio file and run the script, and then: ERROR: File supplied on command line; use -s, -pacbio-raw, -pacbio-corrected, -nanopore-raw, or -nanopore-corrected. Have anyone experienced this before or have a explanation?

assembly canu
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updated by lagartija lagartija • written by knudnornielsen • knudnornielsen

I've never used CANU, but have you tried adding the flag "-pacbio-raw"?

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written by Brian Bushnell Brian Bushnell

1 answer

I use these flags for the inputs and it works. Are you sure that you have correctly downloaded the fastq or fasta file ? Can you copy your entire canu command so we can see ?

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written by lagartija lagartija

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