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Cannot calculate order statistic on object with 3 columns in R

I have 3 .CEL files which are replicates. Im trying to get the diffrentially expressed genes between them but when I reach this line : "celfiles.filtered <- nsFilter(celfiles.normalise, require.entrez=TRUE, remove.dupEntrez=TRUE)"

I get this error: " Error in rowQ(eSet, floor(0.25 * numSamp)) : cannot calculate order statistic on object with 3 columns".

I'm trying to filter the probes after normalizing them to proceed to analyze them using statistical values.

I tried searching for this error but I couldn't reach anywhere so please any help.

r microarray statistics
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written by user_g • user_g

what does celfiles.normalise contain and what is class and structure of celfiles.normalise?

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written by cpad0112 cpad0112

Perhaps you should only have row.names and values, for each file? How are your files composed, what do the columns contain?

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written by Matteo Schiavinato Matteo Schiavinato

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