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Which is most reliable free ware chromatogram viewer?

Dear all I sequenced my gene and I received my sequenced data in abi formate. Now there are a large number of chromatogram viewer. Any one suggest please such a tool that not only find mutations in a sequence but also Amino acid change with position.

sequencing

2 answers

Not sure if most reliable but they are both free novoSNP or GeneScreen.

I like using benchling. You can paste in your expected sequence, then align any number of .abi files to it. I typically use the MAFFT option, but increase the refinement iterations to 10.

Not suitable for batch processing, but if you just have a few genes, it's a nice option.

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