Dereplication step leads to increased file size
Hi,
I have done quality filtering step on my paired end data using FaQCs tool (removal on the basis of Q score and adapter ). After this step i have performed dereplication step using PRINSEQ but i have seen that size of file increased(19GB to 21 GB). Please let me know what are the possible causes of this unusal output.
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aditijangid66 •
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It's be better to count the number of reads in the file before and after the PRINSEQ to get some idea about what's going on.
This is commonly the result of using a different gzip compression level.