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Redundancy in Ensembl GTF

Hi All,

I have an Pre!Ensembl GTF file that has redundant features. The file is from a non-model organism and has uniprot IDs from two or more species corresponding to each gene.

I'd like to use the GTF file for counting with HTSeq but most of the reads are classified as ambiguous.

Has anyone encountered this before, any ideas?

Colin

rna-seq

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