Aligning with huge insertion variations
What would be the best way to align multiple gene sequences that all have various large insertions like SINEs and LINEs and transposable elements.. I cannot get a good alignment. What would be the best algorithm, parameters, and platform to accomplish this?
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Here it depends on what you are looking for. If you want a single, semi global alignment (with at most non-overlapping alternatives) then bwa-mem will do the job.
If you want to look at all possible alignments then you will need to use a generic aligner such as lastz or last
consolidating the information out of all the alternatives these tools may produce may be more challenging.
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which software have you tried? I would expect bwa-mem to be able to handle those alignments with the 'SA' tags