Great, thanks Pierre !!!
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Hey,
I am analyzing RNA-seq data and I am interested in duplicated reads. I know I can count number of duplicated reads overall using picard markDuplicates.
samtools view -f 1024 dedup_reads.bam | wc -l
But i am interesting in the distribution of these duplicated. I have both sam and bam files.
Here is simple what i would to have
# total reads #duplicated reads
Gene1 30 10
Gene2 100 20
Gene3 20 0
I googled but i couldn't find any tools, is there any tools, softwares or packages? Or should I implement myself.
awk '{printf("%s:%d-%d\n",$1,int($2)+1,$3);}' input.bed | while read F;
do echo -n "$F " && samtools view -F 4 -c input.bam $F | tr -d '\n' && echo -n " " && samtools view -f 1024 -F 4 -c input.bam $F
done
updated : oopss added $F after each "samtools view"
Great, thanks Pierre !!!
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