Right now I'm trying a general blast for everything and then searching for the matches by taxid or the seqid's. I'm tried other approaches using gilists and downloading genomes and have still not obtained the results I need.
These are the results I receive from the online version when I search for Arsb transcript variant 1, for organism Gallus gallus (taxid: 9031), an e value cut off of 0.00005, on database refseq_genomic
Database: NCBI Genomic Reference Sequences
23,370,420 sequences; 1,019,245,483,979 total letters
Query= NM_005445.3 Homo sapiens structural maintenance of chromosomes 3
(SMC3), mRNA
Length=4131
Score E
Sequences producing significant alignments: (Bits) Value
NC_006093.4 Gallus gallus isolate RJF #256 breed Red Jungle f... 237 3e-59
NT_455898.1 Gallus gallus isolate RJF #256 breed Red Jungle f... 237 3e-59
I am running a command line blast query which to my understanding should have the two lines of results within the output file. These lines are not in the output however
blastn -query mRNAfiles/Arsb -db database/refseq_genomic -out "blast_output.txt" -evalue 0.00001 -outfmt '6 qseqid sseqid pident evalue bitscore score qcovs qcovhsp qcovus staxids ssciname'. //This command should show all results for all organisms
When I grep for the organisms taxid and the refseq ids of the output, I do not find anything
grep '9031' blast_ouput.txt
grep 'NC_006093.4' blast_output.txt
grep 'NT_455898.1' blast_output.txt
0 answers
No answers yet.
Log in to answer this question.
Please search biostars (use google for an external search) next time : Why the local blast and online blast produce different results?
Blast searches are sensitive to the type and size (content) of the database beings searched against along with settings for gap opening/extension penalty. You will have to check all of those things.
I feel like I have been, I guess I haven't?
No problem, sometimes it is hard to find. But this question comes up once in a while.
Hello Jacob!
Questions similar to yours can already be found at:
We have closed your question to allow us to keep similar content in the same thread.
If you disagree with this please tell us why in a reply below. We'll be happy to talk about it.
Cheers!