This is a test version of Biostars. For the public version, visit https://www.biostars.org.
HISAT2 or SALMON AND KALLISTO

What is good for RNASeq analysis, HISAT2 or Salmon and kallisto ? Please advise if other good option is available.

rna-seq

In my sub-field (environmental eukaryotes), Salmon is more popular than the other two, but as others have mentioned below all three are established and respected methods. Salmon and kallisto are extremely similar. I do not have experience with HISAT2.

1 answer

Have you looked at the respective publications/web pages to understand the differences? Since we don't know what your intended application is the answer would be "depends on your needs".

Adding on this, all methods are accepted and well-tested. Each has their individual strengths and weaknesses. Please read benchmarking papers for details.

Log in to answer this question.