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Variant calling following multiple sequence alignment

Hi everyone,

Are there any tools/pipelines available that I can use to call for variants/SNPs following multiple sequence alignment? I need something that can call for variants between the sequences, and not from a reference genome.

Thank you in advance!

snp alignment

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1 answer

Thank you, snp-sites works for my analyses!

great, clicks the green mark on left to accept and close the question please.

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