DNA barcode creation
What is the meaning of: [ , w, number, { in the DNA barcode integrate in the software DNA barcode , and why we found 3 or 4 letters between the bars barcode Firmicutes;Bacilli;Bacillales;Bacillaceae(Family)
[ATCH]T[ATGD][CAGV][CGTB][GCAV][GTK][GATD][TAGD]\w{20,60}[CTGAN][ACGV][GTAD]A[TAW]G[GATD]\w{18,99}[ACM]C[GCAV]ATGC[GAR][TCY][ACM][GACV][CGS][CGAV]\w{10,35}[ACM][CTGB][TCY][GCS][GAR][GCS]ACTG\w{25,55}A[GTK][TACH]A[GTK]GG\w{10,27}[GTAD][ACM][ATW][AGR][GTAD][TCY][CTGB][TGK][GAR][ACGV][CTY]\w{43,46}CTGT[TCY][GAR][TACH][TCGB][ACGV][GAR][GAR]G[ACGV][GCAV][AGR][AGR]\w{10,80}[TCGAN][CTGAN]AC[GCS][AGR]\w{50,80}AT[TACH]A[TCGB]\w{24,34}T[CTY]T[GCS][AGTD]T[GCAV][TAW]G[ATGD][ACM][ACTH]\w{465,476}G[ATW][TCY]GT[TGK]\w{96,98}GGA[ATW]G[GAR]\w{19,28}T[TAW][ATW][TGK][GAR][ATCH]CC\w{8,25}[CTY][AGR][CGS][GAR][TAW][GTK][CGS][TGAD]AC\w{79,108}[GACV][CAM][TCAH][ACGV][GAR][TAW][AGR]
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I am not familiar with the DNA barcode software, but this looks like a (perl) regular expression to me, in which case the interpretation is as follows:
The letters inside the square brackets [] are options that can occur at that position, so for example [ATCH] means that the character at that position could be one of A or T or C or H. \w means a word character, generally taken to be any character from the letters A to Z, a to z, numbers 0 to 9, or the underscore character _. The number or numbers in the curly brackets following the \w specifies how many word characters to expect at that position, for example {20,60} means 20 or more (the minimum) up to a maximum of 60 word characters.
thank you for your reply For the characters, is not a problem, are the letters used for DNA The proposition of the number may be as you explained; Despite that I have not yet understood on what basis they created the barcode
Where did you get the DNA barcode software you mentioned?
Is the work described in the following paper any help?
http://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-9-576
the software was downlaoded from this site http://www.neeri.res.in/DNA_BarID.htm