Why this change in morphology in endothelial colony forming cells (ECFC)?
Dear colleagues, I culture on 2% PAN-biotech gelatine, cord blood MNCs into endothelial colony forming cells (ECFCs), using Endopan 3 kit medium.
For many of my samples, I obtain a change in morphology of my ECFCs during culture, and these "new looking" ECFCs no longer proliferate. The cells now look enlarged , elongated and lose their cobble-stone morphology (see page 47 of the in below) and can not be used for my further purposes. I am worried about this phenomenon and I want to solve and or avoid it.
I thank you in advance for any suggestions as to why this happens and how I can help the situation.
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Hello charlesgwellem!
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