If it is an expression dataset, and not a SNP array, it could indicate whether the signal was above a certain minimum threshold, perhaps after adjusting for background. This would only be used for microarrays, where each call refers to a specific location on the chip.
What is present and absent calling in high-throughput data?
Hi
I have an expression dataset, It has a column entitled absent/present calling (values 0 or 1), I have two questions:
1- Should I treat different on expression columns based on 0 or 1 ?
2- Is this column just used for Microarray or it could be used for RNA-SEQ too?
Thanks.
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mirzaei86.vahid •
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Can you send a few lines of your data set? I guess "absent/present calling" correspond to certain variants (SNP).
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nazaninhoseinkhan
thanks @mastal511, it is expression data. and from 1700 have 0 value from 33K gene ids. So I should cut 0 value?
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mirzaei86.vahid •
dear Nazanin, my data is expression profile.
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