Soap2 index establishing error
Hi there,
i used soap2 to do some alignments of short sequences, according to the soap2 official website i have to make a index first but when i run the below command line on my server,
2bwt-builder /data/database/UCSC/hg19/genome/hg19.fa
i got a error shows that: Parsing FASTA file.. ParseFASTToPacked() : Cannot open annotationFileName!
somebody konws how to handle with this problem? very thx!
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The error seems to be clear. Looks like you need to provide a corresponding annotation file (generally GTF or GFF format).