Hello, I have some samples to realignment and recalibrate them and I need homo sapiens vcf for do that. Could you please help me where …

Hi I have a txt file that contains some variants with specific columns Example from my txt file chr5 140673 rs12516245 A G I want …

With the help of GATK GenomicsDBImport and Genotypegvcf, I have jointly called variants in siblings and done hardfiltering. I am puzzled about how to find …

I have two list, first list contains defined variants (200 variants) and the second list is actually a .vcf file which contains all variants of …

Hello. I'm new to Bioinformatics working on NGS data. Now I'm working on detecting SNP variants causing multiple sclerosis. I selected some genes for target …

The [clinvar FTP site](https://ftp.ncbi.nlm.nih.gov/pub/clinvar/) has two files that contain variant annotations: * "tab_delimited/variant_summary.txt.gz" * "vcf_GRCh37/clinvar.vcf.gz" The summary table contains variants from multiple assemblies. However, if …

Hello All, I have a simulated VCF file and I have simulated fastq file generated using the `varsim` tool. I wanna find the coverage of …

Dear all, I have multiple vcf file and want to extract allele frequency and the hetero- and homozygous call with their count. Could you please …

I just started working with ReadqPCR and NormqPCR packages (Bioconductor) in R. However, I'm stucked 'cause I can't use deltaDeltaCq() function. First things first... 1) …

<p>Hello!</p> <p>I will work with NGS data, but I just know the techniques.</p> <p>I already have the data (HIV data) but I need to understand …