De novo bacteria transcriptome assembly options
Hello, I am relatively new to transcriptome analysis, definitively new to de novo assembly. I wonder if anyone knows of a good option to assemble a bacterial transcriptome in the absence of a good reference genome? So far I have not been able to find a publication on this topic.
Thanks!
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It's relatively easy (compared to eukaryotes). You can use software for DNA assembly but make sure it doesn't assume even coverage. rnaSPAdes or metaSPAdes should work.
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