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Filter hits that aligned concordantly exactly 1 times base on mpq>40

Hello,

I need to get all reads that aligned concordantly exactly 1 times base on mpq>40 from hisat2.

samtools view -b -q 40 -o output.bam alignments.bam

Will this command make a job?

bam samtools

1 answer

I assume "make a job" means "work". Yes, that will work.

Provided samtools was added before view in the command posted :)

yes, sorry, I deleted it accidentally before posting! thanks guys

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