Why did not BLAST show the results that fit the my requirements?
Dear all. Recently, I did a BLAST search with e value of 1e-10 as the threshold. Then,by doing the same BLAST search without setting the threshold, I found some hits, of which the e value was below the threshold (1.6e-16), did not show up in the orginal search. Could you please tell me what happened? feel really confused...
• 1,766 views
•
link
written
by
2351688559 •
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
filtering blastx output file
written by Bushra Bibi
-
interpretation of orthofinder result
written by mxlsherry1992 •
-
What sequence has being read by IGV's 'copy sequence'
written by marongiu.luigi
-
News: -max_target_seqs 1 in NCBI standalone BLAST does not do what you think it does
written by 5heikki
-
BLAST bit-score/score to E-value comparison for HSPs
written by cklinger •
-
Blast 2.28 evalue threshold for saving hits
written by xi100f •
-
E-Value Threshold Analysis
written by caribsim •
-
short protein BLAST
written by biolab
-
Reciprocal blast and protein identification
written by MAPK
-
Why Do Blastall And Blast+ (Blastn) Give Different Results For What I Beleive Is The Same Search?
written by David M
Please provide a reproducible example, blast version, input data, parameters.
How did you do the searches ? What were the parameters you used in both cases ?
I wanted to search homologous of cryptochrome from Neurospora crassa in human's genome. Thus, I used the protein sequence of Neurospora's cryptochrome as the query, and proteins collection of human genome as the databse. All of the parameters were default, except the e value in the first search, which was set to 1e-10.