Does Minia discard singletons?
Hi everyone,
I am working with some Minia assemblies. These were run using the no-length-cutoff parameter. Normally I discard small contigs, but I like to see what comes out before i do.
My question: when using the no-length-cutoff parameter, does Minia keep everything, even the singletons, reads which were not part of any contig?
Just curious.
Thanks for any information.
Stacy
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1 answer
Hi, yes Minia should keep singleton k-mers that were not filtered out by the abundance threshold. You can check this by looking at the length of the smallest contigs.
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