Hello group, I want to predict amino acid sequence of gene after deleting a single nucleotide?
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written
by
inayat45shaikh •
1 answer
Hi Inayat,
I've posted a few links to translation tools below. Get one nucleotide sequence for the full-length open-reading-frame without the deletion, and one with the deletion, preferably in fasta format. Put them both through the translate-tool. Ensure that you have the correct, in-frame start codon. The genetic code does change slightly in some genomes https://en.wikipedia.org/wiki/List_of_genetic_codes
http://web.expasy.org/translate/
https://www.ebi.ac.uk/Tools/st/
http://www.fr33.net/translator.php
http://www.bioinformatics.org/sms2/translate.html
http://bio.lundberg.gu.se/edu/translat.html
http://molbiol-tools.ca/Translation.htm
http://insilico.ehu.es/translate/
http://www.attotron.com/cybertory/analysis/trans.htm
http://www.bioline.com/media/calculator/01_13.html
https://hcv.lanl.gov/content/sequence/TRANSLATE/translate.html
http://biotools.umassmed.edu/cgi-bin/biobin/transeq
http://in-silico.net/tools/biology/sequence_conversion
http://emboss.sourceforge.net/apps/cvs/emboss/apps/transeq.html
http://db.systemsbiology.net:8080/proteomicsToolkit/DNATranslator.html
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Do you mean how to translate nucleotides to protein? transeq of EMBOSS is one way, must be other tools out there.
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