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GSEA - pathway analysis

Hi everybody,

I have a gene list (more than 6000 genes) and I would like to use GSEA, but it will only accept approximately 2990 genes? Does any one know how to fix it? Additionally, I would like compare my gene list with my own defined background, but I not sure how to do it in GSEA...

Thank you,

Best, Martin

gene

How were you using GSEA? Online? You can also download a standalone version, to which you can probably add your own gene lists as well.

1 answer

I have now downloaded the GSEA, but I have some problems in using my gene list. It is in text file. Can someone help me?

Best, Martin

Which problems do you have? Have you read the manual about the formats required to upload files (it can be a bit tricky)?

I have my gene list (only gene names) in a txt-file, but I read that I have to change the format to others. And I can't really figure out, how to do it. When using GSEA online, it was so easy

Which errors do you get? To which format have you tried to convert it? What is you "own defined background" ?

I have some problems in using my gene list.

Hold on, let me get my crystal ball and find out more about those problems.

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