Filtering BLAST output to avoid 100% identical hits
Hello! I'm trying to get blastn to not output results that are 100% identical to my query sequence. The only filter I can find is -perc_identity, but this seems to filter out results below a threshold: I want to do the opposite. Is there a way to define a range of percent identity to output or to limit results to those that fall below a threshold value?
Any ideas or suggestions would be greatly appreciated. Thank you!
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And what is it you are trying to achieve? Sounds like a weird strategy.
Output in tabular format and filter using awk or excel. You should pay attention to the length of alignment as well.