Entering edit mode
7.3 years ago
wmoss
•
0
Hello! I'm trying to get blastn to not output results that are 100% identical to my query sequence. The only filter I can find is -perc_identity, but this seems to filter out results below a threshold: I want to do the opposite. Is there a way to define a range of percent identity to output or to limit results to those that fall below a threshold value?
Any ideas or suggestions would be greatly appreciated. Thank you!
Output in tabular format and filter using awk or excel. You should pay attention to the length of alignment as well.
And what is it you are trying to achieve? Sounds like a weird strategy.