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How to determine homozygous and heterozygous SNP within a genotype

I am trying to understand how can be read a particular locus on both chromosomes when we do sequencing, and calling a particular SNP homozygous or heterozygous.

Like for SNP detection within a DNA againt the reference sequence:

1) We are doing fragmentation of DNA 2) Ligation of adapter and bar code 3) PCR 4) Denaturation 5) Sequencing

So how we can determine that both chromosome segments were sequenced for a particular locus?

snp
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updated by 2nelly 2nelly • written by Bioinfonext Bioinfonext

1 answer

The whole procedure is unbiased. it s practically impossible to sequence only the one allele if you have a good coverage

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written by 2nelly 2nelly

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