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Motif discovery using MEME

So, I was using meme for motif discovery online (web based) and offline both version. I have used for the same set of sequences and I found that the e-values for the same motif sequences varies using meme offline and online version ,technically this shouldn’t be the case, any idea why the values are different??

meme motif conserved domain

The background frequencies used online and offline are same ?

As far as I know it performs denovo motif discovery without any reference , and the e-value states the statistical significance of motif in the given sequence data. I am not sure about the background frequencies you are asking for, can you elaborate? Thanks

1 answer

Is the difference in terms of e-values huge? As far as I remember, the background is calculated by shuffling the sequences in the input, hence you can have a slight difference Are the versions(online and offline) the same?

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