Normalizing with RMA and filtering with mas5calls (affymetrix arrays)
Hi all,
I'm working with affymetrix arrays and I'm wondering if normalizing data with RMA and then filtering expression data with mas5calls (Presence/Absence calls) is a correct methodology. Somebody knows anything about it?
Thank you in advance!
• 2,710 views
•
link
1 answer
Hi,
Yes its perfectly valid method to do so. It helps to remove those 'noisy' probes with low detection level. I have seen people removing probesets showing Absence in at-least 50% of the samples.
• 0 views
•
link
Log in to answer this question.