1.how to calculate dn/ds ratio for list of mrna and lncrna from mouse data against human ?

2.I have used biomart from ensemble and get list of orthologues against my data and dn/ds ratio ? how I can get accumulative result or make accumulative this result from this data like

3.Or its better to do by orthougue seq alignment but in that case it a big task to get orthologue sequences and then alignment for such big data