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Gene cluster identification using gff file

Hello everyone, I have a list of genes. I want to know which of these are separated by nomore than eight genes (not present in the gene list) on genome using a gff file.

gene genome

I guess you will have to writte your own script for that. Using bioperl you can load all thé feature in memory with thé slurp method then you read the features sorted by start from left to right ans do your Cook to detect what you want.

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