This is a test version of Biostars. For the public version, visit https://www.biostars.org.
How can you explain a high signal on a locus, while it's proven it can't be that high when using UCSC

Here is the result of a ChIP-Seq of the factor Ste12 on a yeast chromosome

(Here is the result of a ChIP-Seq of the factor Ste12 on a yeast chromosome)

This is a bonus question in a university homework I am trying to figure out:

We have to explain why the signal of Ste12 is that high on the locus of chrXII:451,512-469,354 coding for ribosomal RNA while we are 100% sure that Ste12 is not more enriched in that region than 10kb in front of the locus.

So far my guess is that it is probably something about the specificity of the antibody used.

chip-seq
• 1,600 views • link
updated by spacemorrissey spacemorrissey • written by monfette.v • monfette.v

1 answer

Look at the input track for that ChIP-seq experiment. My guess is that you will find high signal for that same region in the input track.

• 0 views • link
written by spacemorrissey spacemorrissey

Log in to answer this question.

More posts like this